Open access
Aug 2026
Generation of 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicide-resistant rice (Oryza sativa L.) germplasm based on enhanced DNA shuffling and CRISPR-mediated base editing.
An efficient DNA shuffling method utilizing flanking sequences and segmented amplification to construct random mutation libraries of rice HPPD and maize (Zea mays L.) HPPD genes is developed, enriched the toolbox for rice gene-directed evolution and resistant variety cultivation.
Le Chen, Dongshu Guo, Zhou Yang et al.
· Journal of Advanced Research · 0 citations