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Johannes Broichhagen

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Open access Sep 2026

Nanobodies Equipped with HaloTag Enable Scalable Fluorescence-Lifetime Multiplexing

Fluorescence lifetime (FL) is a robust strategy for multiplexed imaging; however, variability in fluorophores’ local microenvironment when used on affinity probes broadens lifetime distributions, limiting its practical applicability. We create a modular platform for FL encoding with nanobody–HaloTag variants, enabling...

László Albert, Samrat Basak, H. Körner et al. · 0 citations

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