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Jonathan F. Burnett

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Open access Sep 2026

Biochemical characterisation of fungal bioluminescence enzymes reveals substrate inhibition and secondary turnover of 3-hydroxyhispidin by H3H

The fungal bioluminescence pathway enables autonomous light production from caffeic acid and has emerged as a versatile platform for bioimaging and synthetic biology. However, biochemical characterisation of its core enzymes remains limited. Here, we establish recombinant purification and quantitative in vitro characte...

Jonathan F. Burnett, Johannes Lang, A. Tumber et al. · 0 citations

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