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Jun Ren

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Open access Jul 2026

Design guide for synthetic small regulatory RNAs for high-efficiency gene knockdown in bacteria.

A detailed protocol for the design and construction of synthetic sRNAs is provided, detailing key design principles and critical optimization factors, including scaffold selection, target mRNA binding affinity, target mRNA secondary structure, and Hfq expression levels.

Jun Ren, Yu-Kyung Kim, Hyun Jung Nam et al. · Journal of Microbiology · 0 citations