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Author

Jun-Zhou Wu

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Open access Sep 2026

Pyridoxal-phosphate-dependent biosynthesis of aminovaleramide by AvaS in tRNA.

In eubacteria, decoding of isoleucine codon AUA requires a specialized tRNA (tRNAIle2) modified with lysidine (k2C) at the anticodon wobble position (C34), which switches decoding specificity from methionine (AUG) to isoleucine (AUA). Recently, aminovaleramide cytidine (ava2C) was discovered at the same tRNA position i...

Jing-Jing Sun, Jun-Zhou Wu, Yi-Feng Yuan et al. · 0 citations
Open access Aug 2026

Ion-Pair-Free Capillary HILIC-MS for Sensitive Nucleic Acid Analysis and RNA Modification Mapping

Mass spectrometry (MS)-based nucleic acid analysis provides direct chemical evidence for oligonucleotide sequence, composition, and modifications. However, oligonucleotide LC-MS analysis commonly relies on ion-pairing reversed-phase liquid chromatography (IP-RPLC). Although IP-RPLC provides strong retention and high-re...

Jun-Zhou Wu, Rana Togay, Jing-Jing Sun et al. · 0 citations
Open access Aug 2026

Quantitative RNA modification mapping by mass spectrometry with isobaric tags and nucleobase fragment analysis

The authors develop proteomics-style, RNA-specific isobaric tandem mass tags for quantitative RNA modification mapping by mass spectrometry, revealing condition-dependent changes and interdependencies among modification pathways.

Jun-Zhou Wu, Jing-Jing Sun, Yi-Feng Yuan et al. · 0 citations

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