Recursive self-improvement (RSI) requires AI systems that improve the process of building AI (i.e., AI4AI); machine learning engineering (MLE) offers a concrete, executable testbed for studying this capability. We introduce OpenMLE, an open full-stack system for RSI research in MLE, spanning verifiable task environments with execution feedback (OpenMLE-Gym), operator learning (OpenMLE-RL), and long-horizon search (OpenMLE-Evo). On this stack we post-train Frontis-MA1 (35B) as a meta-evolution agent for MLE, aligning post-training and inference around four atomic program-evolution operators (Draft, Improve, Debug, Crossover): the same operators are trained via execution-grounded SFT and RL on data deduplicated against all evaluation benchmarks, then composed into long-horizon search, coupling learning and evolution in a single loop. On MLE-Bench Lite under a 12-hour per-task budget on one RTX 4090 capped at 12 GB VRAM, Frontis-MA1 (35B) improves Medal Average from 39.39% to 60.61% over its base model with OpenMLE-Evo, and reaches 71.21% with OpenMLE-Evo-Max (benchmark-independent experience priors and asynchronous search), exceeding GPT-5.5 + Codex and approaching GPT-5.6 Sol and the 2.8T Kimi K3. On held-out NatureBench Lite, both components transfer: with the framework fixed, swapping in the trained model raises Match-SOTA from 50% to 70%; with the model fixed, swapping in OpenMLE-Evo raises it from 20% to 50%. We release the model weights and the full OpenMLE stack to enable reproducible research on executable AI4AI toward RSI. Code: https://github.com/FrontisAI/OpenRSI
Junlin Yang, Che Jiang, Yu Fu et al.· arXiv.org· 3 citations
Riboflavin is an essential nutrient widely used in food, feed, and pharmaceutical industries, yet efficient screening of high-yield microbial strains remains a major bottleneck in industrial production. Here, we developed an integrated strategy combining dual selective pressure, optimized microplate fermentation, and stepwise high-throughput screening to accelerate the isolation of riboflavin-overproducing Bacillus subtilis mutants. A dual selective pressure system employing phosphonoarginine-5-amino-6-(d-ribosylamino)uracil (Arp) and the GTP analog 8-azaguanine (8-AZG) enhanced the positive mutation rate. Optimization of microplate culture enabled strong correlation across 96-well, 24-well, and shake-flask fermentations. Stepwise screening further enriched stable high producers, achieving a final positive rate of 21.4%. The best mutant, Hs-G87, produced 4.1 g/L riboflavin in shake flasks and 32.1 g/L in a 5 L bioreactor (yield of 0.11 g/g glucose; productivity of 0.594 g/L/h), demonstrating strong industrial potential.
Fangyu Zhang, Guangqing Du, Miaomiao Xia et al.· Journal of Agricultural and...· 0 citations
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