Open access
Aug 2026
Efficient targeted gene knock-in in Ulva using Cas9 RNPs and long single-stranded DNA donors
This study establishes a streamlined and highly efficient framework for precise insertional mutagenesis and double-mutant generation in Ulva, thereby expanding the genetic engineering toolkit for this macroalga.
K. Ichihara, Chikako Nagasato, T. Yamazaki et al.
· Phycology Journal · 0 citations