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K. Onishi

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Open access Sep 2026

Visualizing structural dynamics during nuclease activation across evolutionarily diverse IscB and CRISPR-Cas9 enzymes

Abstract Cas9 nucleases of CRISPR-Cas adaptive immune systems are programmable RNA-guided DNA endonucleases that evolved from the transposon-associated IscB enzymes. Comparative structural studies have revealed substantial architectural elaboration during this evolutionary transition, including the replacement of the l...

O. Nureki, K. Onishi, Yuta Shuto et al. · 0 citations

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