K. Rasal

Effect of GnRH analogue on steroidogenesis in the teleost fish striped murrel (Channa striata)

Design and in silico validation of donor DNA for RNA-guided recombinase-mediated knockout of mstnb gene in Labeo rohita

It is suggested that the engineered mutation is likely to abolish the functional activity of the mstnb gene, thereby providing a validated donor DNA construct for precise RGR-mediated genome editing in L. rohita.

Design and transfection of CRISPR/Cas9 constructs for the myostatin gene in Labeo rohita muscle cells

The study successfully established CRISPR/Cas9 plasmid constructs for the mstnb gene in L. rohita and demonstrated their transfection in LRDM cell line across multiple passages, providing a basis for future studies on genome editing approaches using CRISPR/Cas9 constructs in fish muscle cell lines.