Author

Kaveendya S. Mallikaarachchi

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Open access Jun 2025

Bacterial ribonucleoprotein bodies maintain an acidic pH environment as a mechanism of enzyme regulation

Phase separated biomolecular condensates create subcellular niches, yet their role in client regulation remains unclear. Here, we demonstrate that Bacterial Ribonucleoprotein bodies (BR-bodies) are acidic. Using ratiometric fluorescent probes in vivo, we find BR-bodies exhibit a dense-phase pH of ∼5.1, significantly lo...

Wade E. Schnorr, Moeka Sasazawa, Kathryn G. Dzurik et al. · bioRxiv · 0 citations

Open access Jul 2026

BR-bodies link RNA homeostasis to stress tolerance and intracellular fitness in Brucella

ABSTRACT Bacterial gene expression depends on the coordinated regulation of RNA synthesis, processing, and decay. The conserved RNA degradosome scaffold, ribonuclease E (RNase E), assembles into phase-separated bacterial ribonucleoprotein bodies (BR-bodies) through its C-terminal intrinsically disordered region (IDR)....

K. S. Mallikaarachchi, Rosemary Northcote, Thomas Kim et al. · bioRxiv · 1 citation