Modulating nanoconfined space charge for PCR-free and bisulfite-free detection of cfDNA methylation in ovarian cancer.
Aberrant DNA methylation in circulating cell-free DNA (cfDNA) represents a critical epigenetic biomarker for noninvasive diagnosis of ovarian cancer. However, conventional methodologies are inherently limited by the destructive nature of bisulfite conversion and the operational complexities of PCR thermal cycling. Here...