Kai-Yue Wang

An optimized high-pH solubilization strategy for efficient purification and functional recovery of E. coli-expressed Cry toxins.

An optimized in vitro solubilization and purification protocol utilizing a highly alkaline carbonate buffer to efficiently recover Cry toxins successfully overcomes the technical bottleneck of inefficient inclusion body solubilization during the in vitro preparation of Cry toxins, thereby laying a reliable material fou...