Rapid and Efficient Affinity Isolation of Engineered Extracellular Vesicles Encapsulating Functional Proteins via Internal Twin-Strep Tetraspanin Tagging.
Extracellular vesicles (EVs) are mediators of intercellular communication and promising protein-delivery vehicles, but isolation of sufficiently pure, intact, engineered EVs remains challenging due to abundant cellular and serum-derived contaminants. Here, structure-guided engineering of the canonical EV tetraspanins,...