Photoactivatable NPOM-caged split-activator CRISPR/Cas12a biosensor for amplification-free imaging of deoxynivalenol-induced miR-155 dynamics and lateral-flow sensing.
MicroRNA-155 (miR-155) is an immune-associated small RNA biomarker involved in macrophage responses to environmental stressors such as deoxynivalenol (DON). However, achieving amplification-free miRNA sensing with low background and precise temporal regulation remains challenging. Here, we develop a photoactivatable sp...