Kensho Kawagoe

Rapid and efficient generation of multigene-knockout THP-1 cells using a simple CRISPR-Cas9 protocol

THP-1, a widely used human monocytic leukemia cell line, has been described as one of the hard-to-transfect suspension cell lines. Here, we present a rapid and efficient method for generating multiplex gene knockout cells from THP-1 using a simple co-transfection strategy based on the CRISPR-Cas9 system. In this method...