Enhancing the 12S rRNA reference library of South African chondrichthyans to aid in eDNA metabarcoding
The rapid expansion of environmental DNA (eDNA)-based biodiversity monitoring has heightened the need for accurate and well-curated taxonomic reference databases. Although mitochondrial cytochrome oxidase I (COI) is widely used, the mitochondrial 12S ribosomal RNA gene (12S rRNA) has increasingly demonstrated strong ta...