Rational design of chimeric polyhydroxyalkanoate synthases suggests a conserved N-terminal domain-to-LID region network influencing 3HHx incorporation and granule morphology.
The global microplastic crisis and the suboptimal material properties of biodegradable polyhydroxyalkanoates (PHAs) necessitate the engineering of efficient PHA synthases (PhaCs). However, the narrow substrate specificity of natural PhaCs limits the production of tailored PHA copolymers. Here we report a bioinformatics...