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An Electroporation-Based Protocol for Ex Vivo Base Editing: From Design to Quantitative Assessment.
Base editing is a CRISPR variant approach that enables single-nucleotide conversions without generating double-strand breaks. Cytosine and adenine base editors mediate C•G to T•A and A•T to G•C transitions, respectively, by coupling a deaminase to a catalytically impaired Cas9, a modified nuclease that lacks DNA cleava...
Irene Peña-Gutiérrez, Davide Mazzeo, A. Bassons-Bascuñana et al.
· Methods in molecular biology · 0 citations