Author

L. Swint-Kruse

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Open access Aug 2026

Amber Suppressor tRNA-Based Mutagenesis for Positional Semi-Saturated Mutagenesis with Natural Amino Acid Substitutions: An Approach for Mapping Positional Contributions to Protein Function

There is a growing interest in methods that illuminate the contributions of individual positions to a protein’s function by site-saturating mutagenesis. However, a commonly used approach for assessing variant libraries with deep mutational scanning relies on readouts of biological fitness, which is influenced by many p...

Pierce T. O'Neil, Tonya N. Zeczycki, Kyung-Tae Park et al. · Biophysica · 0 citations

Open access Aug 2026

RheoScale 2.0: Revealing the hidden roles of protein positions via substitution patterns

A central challenge in molecular biology is understanding how amino acid substitutions modulate various features of protein function and stability. To illuminate the complexities of this relationship, high-throughput (HTP) assays are increasingly used to assess site-saturating mutagenesis libraries. A common downstream...

David H. Liu, Shwetha Sreenivasan, Carter J. Gray et al. · bioRxiv · 0 citations