Metal Coordination Dynamics Governs Selective Halogenation in α-KG/Fe-Dependent Halogenase SyrB2.
α-Ketoglutarate (α-KG)-dependent nonheme iron enzymes catalyze a diverse array of oxidative transformations essential for natural product biosynthesis. However, the mechanism by which α-KG/Fe-dependent halogenases achieve selective halogenation while circumventing the thermodynamically favored hydroxylation pathway rem...