PEG 1000 as a molecular switch: Concentration-dependent aggregation and refolding in a non-aggregation-prone β-barrel protein.
Macromolecular crowding significantly influences protein folding, stability, and aggregation within cellular environments. Here, Cellular Retinoic Acid Binding Protein I (CRABP I), a highly stable β-barrel protein with low intrinsic aggregation propensity, was employed to investigate the concentration-dependent effects...