Author

Li-Hua Ding

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Aug 2026

Orthogonal Cas13a/Cas12a cascade for one-pot amplification-free detection of miRNA-21.

MicroRNAs (miRNAs) are promising biomarkers for clinical diagnosis and disease monitoring. However, current CRISPR/Cas-based miRNA sensors generally require reverse transcription or nucleic acid amplification to improve sensitivity, which complicates the workflow and increases the risk of contamination, nonspecific amp...

Jing Yuan, Meng Shen, Li-Hua Ding et al. · Talanta: The International J... · 0 citations

Review Jul 2026

Integrating CRISPR/Cas Biosensors with Advanced Platforms: A Holistic Path Toward Preamplification-free, Multiplexed, and Continuous Molecular Monitoring.

This review discusses how nanomaterials facilitate signal generation and transduction, how microfluidics automates, multiplexes, and miniaturizes "all-in-one" devices, and how orthogonal CRISPR systems can enable robust multiplexing.

C. Effah, Xinyu Li, Qi-Meng Zhang et al. · ACS Sensors · 1 citation