Liang-Jun Li

Structure-Guided Remodeling of Multi-Substrate Binding Pocket in Nicotinamide Mononucleotide Adenylyltransferase for Efficient NAD+ Synthesis

The rational engineering of enzymes that coordinate multiple substrates within a confined active site remains a major challenge in biocatalysis. Here, we present a structure-guided dual-cluster engineering strategy that exploits low epistatic coupling among conserved residues to independently tune electronic and geom...