Spatiotemporal Sequential Fluorescence and Electrochemistry Bimodal Quantitation of Enzyme Activity in a Living Cell.
Accurate spatiotemporal single-cell quantification of intracellular enzyme activity is critical for understanding cellular heterogeneity and disease screening. However, current methods relying on single-signal modalities lack precision, and spatiotemporal multimodal strategies remain unexplored. Here, we develop a spat...