Luke P. Lee

Ultrasound-enhanced exosome secretion and antibody-free SERS profiling for Alzheimer’s disease via iPSC-derived cortical organoids

Exosomes offer a promising vehicle for noninvasive diagnosis of neurodegenerative diseases by carrying multiple biomarkers across the blood-brain barrier. However, traditional exosome analysis often misses subtle genotype-specific variations because of weak signals from a few exosomes, reliance on markers, and limited...

Origins and Proteomic Dynamics of Human Induced Pluripotent Stem Cell-Derived Extracellular Vesicles along the Temporal Trajectory of Cardiomyocyte Differentiation

Stem-cell-derived extracellular vesicles (EVs) that carry bioactive proteins and nucleic acids reflecting the parental cells offer a promising route for cell-free cardiac regenerative medicine. However, the origin, composition, and function of EVs derived from differentiating human induced pluripotent stem cells (iPS...

Inhalable mucus-penetrating and senescence-targeted nanoliposomes reverse senescence to overcome chemoresistance for enhanced lung cancer therapy.

Lung cancer therapy is severely hindered by the pulmonary mucus barrier and chemotherapy resistance driven by therapy-induced senescence (TIS) following DNA-damaging agents. Herein, we report an inhalable, mucus-penetrating nanoliposomes (DR@DPPC-Apt) engineered by incorporating an L1 cell adhesion molecule (L1CAM)-tar...

Cellular and In Vivo Fate of DNA Nanostructures.

DNA nanotechnology is a cutting-edge discipline that exploits the principle of complementary base pairing to direct the self-assembly of precisely defined nanostructures. This review systematically surveys the fundamental concepts, assembly strategies, and biomedical applications of DNA nanostructures, with a particula...

Multidimensional Regulatory Network of Cellular Senescence: From Mechanisms to Theranostics.

Glioblastoma Invasion Remodels Neural Circuits and Drives Persistent GABAergic Dysfunction in Human Brain Organoids

This human iPSC-derived tumor–brain organoid platform provides a reliable and scalable system for studying complex tumor-neural interactions and exploring therapeutic approaches that aim to eliminate the tumor while preserving neural function.