Author

M. Claassen

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Open access Jan 2025

Accurate quantification of spliced and unspliced transcripts for single-cell RNA sequencing with tidesurf

Tidesurf, a command line tool for the quantification of spliced and unspliced transcript molecules from scRNA-seq libraries, is presented, showing the accuracy on various datasets generated with either 3’ or 5’ chemistry, whereas velocyto’s results are highly erroneous for the latter.

Jan T. Schleicher, Doreen Klingler, M. Claassen · bioRxiv · 1 citation