Prokaryotic expression and characterization of IL-21 and its combined effect with IL-2 as an efficacious chimeric drug for cancer therapy
Human interleukin-2 (IL-2) and interleukin-21 (IL-21) are pleiotropic cytokines crucial for immune cell activation and proliferation. This study reports the cloning, expression, and characterization of IL-21 in E. coli system using the pET28a (+) vector. Optimal induction with 0.1 mM IPTG yielded ~ 40% IL-21 expr...