Spontaneous Refolding of Escherichia coli Methionine Adenosyltransferase (MetK) Is Extremely Slow, Indicative of Chaperone Dependence.
Escherichia coli methionine adenosyltransferase (MetK) catalyzes the synthesis of S-adenosylmethionine from ATP and l-methionine and is essential for cellular metabolism. To gain insight into its conformational stability and folding behavior in vitro, we investigated the unfolding and refolding of MetK using guanidine...