Cells must prevent incompletely spliced transcripts from entering the cytoplasm, yet how splicing status is recognized and converted into selective nuclear retention remains unclear. Here, we identify a network of RNA-binding proteins that retain pre-mRNPs within nuclear speckles. Proteomic analysis of splicing interme...
K. Dörner, K. Kurth, Nicole Beuret et al.· bioRxiv· 0 citations
Biomolecular condensation emerges from networks of multivalent interactions that respond collectively to molecular composition and environmental conditions. Here, we establish a sequential Design of Experiments (DoE) framework to systematically and efficiently characterise multidimensional condensation landscapes. Appl...
Matej Siketanc, Ferdinand Weidner, Casimir Nüsperling et al.· bioRxiv· 0 citations
Compartmentalisation enables cells to spatially organise essential biochemical processes. This is exemplified by the eukaryotic nucleolus, which concentrates the machinery for ribosome biogenesis1,2. Bacteria are generally thought to lack an equivalent compartment. Here we discover a nucleolus-like condensate in Escher...
M. Gut, K. Dörner, Sebastian Abegg et al.· bioRxiv· 0 citations
This work identifies PAIP1 as a metazoan cofactor of the mRNA export ATPase DDX19, and identifies an export-coupled mRNP maturation pathway linking PABP exchange to downstream mRNA metabolism.
D. Overwijn, K. Dörner, Caroline de Almeida et al.· bioRxiv· 0 citations
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