Engineering the substrate-binding domain of chitinase for enhanced bioconversion of crustacean shell waste in probiotic feed fermentation.
BACKGROUND Crustacean shell waste, generating 8 million tons annually from global aquaculture, represents an underutilized chitin-rich feedstock. However, the high crystallinity of native α-chitin currently prevents cost-effective enzymatic valorization, limiting its conversion into value-added products for feed applic...