Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) primarily targets the respiratory epithelium, yet severe disease features diffuse lung injury and hyperinflammatory syndromes driven by dysregulated immune activation. Emerging evidence indicates that resident and infiltrating immune cells in the lung can enc...
Yusuke Matsui, R. Suryawanshi, Mauricio Montano et al.· Science Advances· 0 citations
Abstract CRISPR diagnostics enable sensitive detection of infectious diseases, with the RNA endonuclease Cas13a providing specific, amplification-free RNA detection through collateral trans-cleavage of fluorescent reporters. However, background cleavage from unbound enzyme, contaminating nucleases, and unsynchronized i...
Carlos F. Ng, Deepak Krishnamurthy, Andres Dextre et al.· Nucleic Acids Research· 0 citations
A multi-scale analytical framework integrating nanoscale enzyme kinetics, microscale diffusion constraints, and macroscale assay parameters is presented that is used to develop a bead-based Cas13a assay where the bead becomes fluorescent when the target RNA for that bead is detected.
A. Bhuiya, Siddhansh Agarwal, Carlos F. Ng et al.· bioRxiv· 0 citations
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