Tauopathies are defined by the accumulation of filamentous tau assemblies that adopt disease-specific molecular conformations, or tau strains. Although tau is extensively hyperphosphorylated in Alzheimer’s disease, how phosphorylation patterns influence tau folding and strain selection remains unclear. Here, we show that site-specific phosphorylation of 0N3R tau by extracellular signal-regulated kinase 2 (ERK2), occurring predominantly within the proline-rich region and largely excluding the microtubule-binding domain, is sufficient to direct tau assembly into a structurally homogeneous fibril conformation. Residue-resolved NMR spectroscopy identifies a defined and quantifiable ERK2 phosphorylation pattern in the proline-rich region of tau. Cryo-electron microscopy at 3.0 Å resolution reveals that ERK2-phosphorylated 3R tau assembles into filaments with an ordered cross-β fold adopting an Alzheimer’s disease PHF fold, despite the absence of phospho-sites within the fibril core. These filaments exhibit robust seeding activity in tau biosensor cells. Together, these results demonstrate that kinase-specific phosphorylation outside the amyloid core can be sufficient to bias tau toward a defined fibril structure, establishing a direct mechanistic link between kinase specificity, post-translational modification, and tau strain formation.
Phenol-soluble modulin α1 (PSMα1) is a cytolytic peptide secreted by Staphylococcus aureus that contributes to host-cell damage and biofilm stability, yet the relationship between its assembly behavior and function remains incompletely understood. Here, we combine cellular assays, molecular spectroscopy, and high-resolution structural approaches to elucidate how environmental conditions govern PSMα1 activity and supramolecular organization. Live-cell imaging and cytotoxicity assays show that PSMα1 accumulates at the plasma membrane of human cells prior to membrane permeabilization, linking membrane association to cytotoxic outcomes. This process is strongly attenuated by epigallocatechin gallate (EGCG). Cryogenic electron microscopy (cryo-EM) reveals two polymorphic canonical amyloid fibril architectures that share a conserved hydrophobic core and protofilament interface. In parallel, we identify pH as a key determinant of PSMα1 assembly pathways, driving a bifurcation between cross-β amyloid fibrils at extreme acidic and alkaline conditions and heterogeneous, long-lived, thermally stable α-helical nanotubular assemblies at acidic, near-neutral, and slightly alkaline conditions, which act as transient intermediates under highly acidic conditions. Together, these findings demonstrate that PSMα1 is not a single amyloid structure but a condition-dependent structural system in which environmental cues dictate assembly, membrane interaction, and cytotoxic function. This work provides a framework for understanding how polymorphic assembly of bacterial virulence peptides interfaces with host-cell interactions and suggests new avenues for targeting PSM-mediated pathogenicity. Statement of significance Staphylococcus aureus causes severe infections and uses the peptide PSMα1 to damage host cells and strengthen protective biofilms. Like many disease-associated proteins, PSMα1 self-assembles into amyloid fibrils, though their role in virulence remains unclear. We show that PSMα1 does not adopt a single architecture. Instead, environmental changes, such as those at infection sites, drive the peptide into distinct assemblies, including cross-β amyloid fibrils and unexpectedly stable nanotubes with α-helical features. Live-cell imaging shows PSMα1 accumulates at the plasma membrane before cell death, and that epigallocatechin gallate reduces membrane association and toxicity. These findings show that bacterial virulence can be regulated through environmentally controlled transitions between protein assemblies, identifying membrane accumulation as a promising anti-virulence target.
Sambhasan Banerjee, V. Skoryk, Bader Rayan et al.· bioRxiv· 0 citations