Liquid-liquid phase separation (LLPS) drives the formation of membraneless organelles that are crucial for the spatial organization of eukaryotic cells. Given the critical role of biomolecular condensates in health and disease, it is important to gain a quantitative understanding of this phenomenon. Here, we summarize...
Johannes Schmoll, Nina C. Kathe, Mihajlo Novakovic et al.· Current Opinion in Structura...· 0 citations
Liquid-liquid phase separation (LLPS) is a fundamental mechanism that drives biomolecular assembly. The concentration of biomolecules within phase-separated condensates is a critical parameter that reflects their physical state, modulates their biological functions, and provides insights into their formation and aging....
Jiu-Kai Tang, Nina Chiara Kathe, Sung-Sik Lee et al.· ACS Sensors· 0 citations
A detailed, step-by-step protocol for LMA stock preparation, droplet formation, and embedding is presented, and examples of specific downstream biophysical readouts are provided, showing that specific droplet properties are preserved after LMA embedding in the representative systems tested.
Nina C. Kathe, L. Emmanouilidis, Mihajlo Novakovic et al.· Journal of Visualized Experi...· 1 citation
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