Ming-Hua Ji

Computational simulation to improve the activity of a heterologous feruloyl esterase secreted by Bacillus subtilis.

Alanine scanning confirmed His144 and His274 as essential catalytic residues, indicating that targeted modification of the substrate-binding pocket is a viable strategy to improve FAEs for lignocellulose bioconversion.

High-level surfactin production by Bacillus subtilis through integrated metabolic engineering and bioprocess optimization.

The integration of multi-level strain engineering with fermentation optimization thus established B. subtilis ATCC 6051a as an effective platform for high-level surfactin production and provides a practical framework for the development of efficient microbial surfactin production systems.

Recent Advances and Future Perspectives in the Biomanufacturing of Lipopeptides: From Strain Engineering to Industrial Applications.