Open access
Sep 2026
Repeat region engineering of Cas13a crRNA enables conformational gating-based autocatalytic CRISPR biosensing
The findings redefine the crRNA scaffold as a versatile signaling node and provide a generalizable framework for developing high-sensitivity, self-amplifying CRISPR biosensors through topology-driven guide RNA engineering.
Wei Ding, Xiao-Min Yang, Yu-Tong Yang et al.
· Nucleic Acids Research · 0 citations