Author

Mohan Huang

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Review Open access Oct 2026

Synthetic Biology and Systems Metabolic Engineering of Aminoglycoside Antibiotics: From Biosynthetic Pathways to Resistance-Evading and Low-Toxicity Design

Aminoglycoside antibiotics are important therapeutic agents in the clinical treatment of Gram-negative bacterial infections; however, the spread of antimicrobial resistance, together with dose-limiting ototoxicity and nephrotoxicity side effects, substantially restricts their clinical utility. Recent advances in synthe...

Shang-Ye Chu, Yu-Qing Chu, Mo-Han Huang et al. · Microorganisms · 0 citations

Jul 2026

TOPS-CRISPR: Thermally-regulated and oligonucleotide-mediated one-pot CRISPR-Cas12a assay for ultra-sensitive and rapid on-site diagnostics.

A Thermally regulated, Oligonucleotide-mediated one-Pot System for CRISPR-Cas12a (TOPS-CRISPR), which employs a programmable inhibition strategy based on complementary RNA blockers with tunable length and binding sites, enabling efficient and reversible steric inhibition of the LbCas12a-crRNA ribonucleoprotein (RNP) co...

Shusen Ji, Bin Wang, Yi Yan et al. · Biosensors & bioelectronics · 0 citations