Balancing Precursor Supply and Enhancing Enzyme Solubility for Efficient Production of ( S )-Reticuline in Escherichia coli
Benzylisoquinoline alkaloids (BIAs) are pharmacologically valuable natural products, yet their microbial production in Escherichia coli is hindered by two major bottlenecks: (1) imbalanced metabolic flux between the two upstream precursors, 3,4-dihydroxyphenylacetaldehyde (3,4-DHPAA) and dopamine, and (2) poor solubl...