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N. Kruglova

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Open access Jun 2026

Production of virus-like particles with AsCas12a nuclease and CMV-driven crRNA for mammalian genome editing

A detailed protocol is described for a small-scale production of AsCas12a-VLPs using three distinct transfection methods and a large-scale production of VLPs using calcium-phosphate transfection, showing that both production scales yield comparable nuclease loading into VLPs and similar editing efficiencies.

N. Kruglova, S. E. Borovikova, M. Shepelev · 0 citations