Review
Open access
Jul 2026
Comparative Quantitative Analysis of Kcat Modulation in Laccases Engineered by Rational, Semi-Rational, and Directed Evolution Approaches
Direct evolution combined with rational and semi-rational design yielded the greatest improvements, reaching kcat values up to 1328.8 s−1 for ABTS, establishing it as the primary performance metric for evaluating laccase engineering outcomes.
Alan Rodríguez-Enríquez, N. Rosas-Murrieta, Eduardo Torres
· Catalysts · 0 citations