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Author

Nanami Hashimoto

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Open access Oct 2026

De Novo Isolation of an Antigen-Binding Antibody by Targeted CDR-H3 Diversification of a Germline-Derived Scaffold in a Site-Specifically Integrated Mammalian Display Platform: A Proof-of-Principle Study

Background/Objectives: Mammalian display enables direct screening of full-length IgG antibodies, but genetically controlled library construction remains technically demanding. Here, we developed a mammalian display platform in which a target-naïve ΔDH antibody scaffold is first established at a defined human artificial...

Ryoichi Sakurai, Nanami Hashimoto, Narumi Uno et al. · 0 citations

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