Cell Disruption and RNA Extraction from Rhodococcus and Gordonia Bacteria Grown in the Presence of Hydrophobic Organic Compounds and Heavy Metal Salts
Non-pathogenic actinomycetes play a key role in the degradation of xenobiotics and the biotransformation of complex organic compounds. Studying gene expression in these bacteria can be challenging due to the rigid cell wall that hinders RNA extraction. Here we present the first comprehensive study of total RNA isolatio...