Neil J. Oldham

Carbene Footprinting and Mass Spectrometry Capture Global Conformational Changes in Elongation Factor-Tu Induced by Antibiotic Binding

Carbene footprinting methodology, in combination with mass spectrometry, was employed to study the interactions of bacterial elongation factor-thermo-unstable (EF-Tu) with two antibiotics. EF-Tu, a GTPase, delivers aa-tRNA to the ribosome during protein synthesis where it undergoes a significant conformational switch...

Hierarchical regulation of oxygen-sensing through coupled transducers in Arabidopsis thaliana

The sensing of molecular oxygen is an essential component of development and environmental response in both animals and plants. Few substrates of the plant oxygen-sensing PLANT CYSTEINE OXIDASE (PCO) N-degron pathway have been identified. Here we show that HYPOXIA TRANSDUCER1 (HYT1, previously named HYPOXIA-RESPONS...

Functional diversification of UBP6 in plant immunity through N-degron pathway regulation