Disease-associated missense mutations are well known to affect protein turnover by either impairing substrate-ubiquitin ligase interactions or inducing misfolding-triggered protein clearance. Whether such mutations can drive gain-of-function in substrate-selective protein degradation remains under-explored. Here, we de...
Bai-Yun Wang, Chi-Wei Yeh, Hsuan-Mei Lin et al.· bioRxiv· 0 citations
The plant defence hormone salicylic acid (SA) triggers systemic acquired resistance by signalling through its receptor, NON-EXPRESSOR OF PATHOGENESIS-RELATED GENE 1 (NPR1), leading to widespread transcriptional reprogramming1,2. However, the molecular mechanism by which NPR1 senses SA and activates transcription remain...
Song-Wen Zhang, Madeline Gish, Huan Li et al.· Nature· 0 citations
It is found that affinity governs the phase boundary, resistance to chemical perturbation, and molecular mobility of condensates in vitro and in human cells and is shown to be a quantitative determinant of condensate phase behavior, internal dynamics and biochemical output.
Andres Reyna, Madyson O. Briggs, Alexander F. Russell et al.· bioRxiv· 0 citations
Protein-protein interactions (PPIs) are fundamental to cellular signaling networks, yet many remain undetected due to technical limitations of individual affinity purification approaches. To address this, we systematically mapped the interaction landscapes of six regulatory proteins involved in cell proliferation, immu...
