Olivier Haccard

Synthetic gRNA-mediated multiplex CRISPR enables the generation of translucent F0 Xenopus laevis for in vivo imaging

Transparent model organisms are invaluable for live imaging, yet generating them remains challenging. Here, we present a robust strategy to produce translucent Xenopus laevis , enabling non-invasive, deep-tissue imaging in intact organisms. Using CRISPR/Cas9 technology, we generated quadruple knockout animals tar...