Targeted Quantitative Analysis of Polar and Phosphorylated Intermediates of Algal and Plant Primary Carbon Metabolism Using an Optimized HILIC-Amide Tandem MS Workflow
Central carbon metabolism coordinates energy production and carbon skeleton allocation across photosynthetic and heterotrophic organisms. Here, we established an optimized targeted metabolomics workflow for Chlamydomonas reinhardtii and Arabidopsis thaliana using a single-run HILIC-MS/MS assay with alternating positive...