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Author

P. Morsomme

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Open access Sep 2026

Cell-free membrane systems reveal electrostatic control of ion transport in TMEM165 and disease-associated CaCA2 variants

Ion transport across intracellular membranes is essential for cellular homeostasis, yet organellar transport mechanisms remain difficult to resolve in native cells. Here, we establish a cell-free membrane system to interrogate full-length intracellular transporters under defined chemical conditions. Using cell-free pro...

Ekaterina Selivanovitch, Alexis R. Ostwalt, Angelica Byra et al. · 0 citations
Open access Aug 2026

A cleavable signal peptide controls the topology and Golgi targeting of the membrane protein TMEM165

TMEM165 is a Golgi-resident multi-pass membrane protein involved in divalent cation homeostasis and associated with congenital disorders of glycosylation, yet its N-terminal biogenesis has remained unresolved. Here, we demonstrate that TMEM165 contains a functional cleavable signal peptide required for correct Golgi ta...

Marie-Odile Velings, Romain Simar, Andréa Bleret et al. · 0 citations

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