Cell-Friendly 3D Bioprinting of Gelatin-Norbornene Bioink via Thiol-Ene Crosslinking Enabled by Interfacial DTT Delivery from Alternating Support Layers.
Thiol-ene gelatin-based hydrogels, particularly gelatin-norbornene (GelNB)-based hydrogels, are promising for extrusion-based three-dimensional (3D) bioprinting owing to their rapid oxygen-insensitive crosslinking and excellent biocompatibility. Dithiothreitol (DTT) is commonly used as a thiol crosslinker because of it...