Pamela Lojan-Cueva

Surveying armadillo and bat trypanosomes by DNA metabarcoding with Oxford Nanopore Technologies sequencing: the importance of fine-tuning parameters to identify mixed infections

The findings indicate that active multi-strain Trypanosoma coinfections are rare, aligning with the principle of competitive exclusion, and highlight the necessity of applying rigorous bioinformatic filters to accurately evaluate host-parasite interactions and avoid overestimating diversity metrics.