Engineering a modular p2GUS expression system for enhanced Ni-binding protein production in Escherichia coli
Pedro Ferro-Gallego, L. Domínguez-Gerpe
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Open access
Aug 2026
Proof-of-concept validation of p2GUS, a modular expression system for enhanced Ni-binding purification of functional recombinant proteins in Escherichia coli
Protein expression and purification remain critical steps in basic research, biotechnology, and industrial applications. Here, p2GUS, a modular Escherichia coli expression system generated through targeted modification of Precursor 1, a parental pBAD-derived vector, is described. The principal feature is a tand...
Pedro Ferro-Gallego, Lourdes Domínguez-Gerpe
· Applied Microbiology and Bio... · 0 citations