Author

Pierre Santucci

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Open access Oct 2026

Mycobacterium tuberculosis gene expression dynamics in host environments with single-cell resolution.

Visualizing when and where Mycobacterium tuberculosis (Mtb) genes are expressed in the host cell is crucial to understand tuberculosis heterogeneity. However, imaging approaches for studying spatiotemporal gene expression in Mtb remain limited. Here, we developed a dual-fluorophore Mtb reporter system that enables high...

Beren Aylan, Laure Botella, Pierre Santucci et al. · Cell Reports Methods · 0 citations

Open access Oct 2026

Development of inducible genetic systems to finely tune efficacy of InhA-targeting anti-tuberculous drugs

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a leading global health threat, especially due to multidrug-resistant strains. The first-line drug isoniazid (INH) and second-line drugs ethionamide (ETH) and prothionamide (PTH), are prodrugs that target the essential enoyl-ACP reductase InhA, a ke...

Eliana Sepulveda Ramos, Maartje Deschutter, V. Point et al. · bioRxiv · 0 citations

Open access Aug 2026

A simple multi-cloning strategy for gene expression and subcellular localization in Mycobacteria.

A streamlined cloning platform for expressing a gene of interest either alone or fused to a monomeric fluorescent protein under the control of two promoters of choice is described, providing a fast and flexible cloning strategy for protein expression and subcellular localization analyses in mycobacteria.

Maartje Deschutter, Eva Beaumale, S. Canaan et al. · Biochimie · 0 citations